Fig. 3. CAI-induced inhibition of store-operated inward calcium currents. (A) HEK-293 cells were pre-incubated with 5 µM CAI (thin trace) or not (CTRL, thick trace). Gaps in the current traces correspond to the time taken to apply voltage ramps to determine current/potential relationships. The addition of 5 µM CAI (horizontal line) did not reduce the amplitude of the plateau phase of the inward current recorded in the control conditions. Whole-cell currents were measured using 250 millisecond voltage steps from a holding potential of 0 to -80 mV delivered every 2 seconds. (B) Mean results from a series of experiments similar to those described in A. Values are the mean±s.e.m.; n=5.