Fig. 7. Confocal microscopy of the CysLT₁ receptor trafficking in intact dU937 cells. Confocal laser scanning microscopy of the subcellular distribution of the CysLT₁ receptors was performed using a specific antibody raised against the C-terminal tail. Cells were fixed with paraformaldehyde and permeabilized with Triton X-100 in basal conditions (control), and after 5 minutes were challenged with the indicated stimuli. Immunocytochemical staining of five representative dU937 cells after stimulation with vehicle (A), 10 nM LTD₄ (B), 100 µM ATP (C), 1 mM UDP (D), 500 nM PMA (E) and 10 nM LTD₄ after a 30 minute pretreatment with 10 µM GFX (F). The experiment shown is representative of at least two others performed.