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Fig. 4. mAKAP RNAi inhibits protein synthesis. To detect new protein synthesis, myocytes were infected with control or mAKAP siRNA-expressing adenovirus. Myocytes were cultured for 18 hours in medium containing [3H]leucine and either no agonist (No Drug), 10 µmol/l Iso, or 100 µmol/l PE and 1 µmol/l propanolol (PE). Total [3H]leucine incorporation was determined. Data are normalized to the control siRNA, untreated sample (bar 1). *P≤0.05; n≤4 independent myocyte preparations.