Fig. 5. mAKAP RNAi inhibits ANF expression. (A) Myocytes were infected with control (a-c) or mAKAP (d-f) siRNA-expressing adenovirus. The cells were treated with 100 µmol/l PE and 1 µmol/l propanolol for 48 hours (a-f) or left untreated (not shown), and then stained with monoclonal mAKAP antibody (red, a,d), rabbit ANF antibody (green, b,e) and Hoechst DNA stain (shown in the merged images in c,f). Bar, 20 µm. (B) The fraction of myocytes showing peri-nuclear, Golgi-type ANF staining is indicated for treated and non-treated myocytes. Data are average expression ± s.e.m. ^*P=0.03; n=4.