Fig. 6. Effect of SV2A and SV2C overexpression on insulin secretion in INS-1E cells. (A) Western blotting analysis of overexpressed SV2A and -C in INS-1E cells using the antibody against SV2 (top panel). INS-1E cells infected with the control AdCALacZ (LacZ), AdCASV2A (SV2A) or AdCASV2C (SV2C) adenoviruses, were incubated at basal 2.5 mM glucose (glc) and stimulated for 15 minutes with 30 mM KCl, or for 30 minutes with 15 mM glucose. Data are the mean±s.e. of five independent experiments. Asterisks indicate significant differences from levels in the LacZ control at P<0.05. (B) Western blotting of exogenous SV2A and -C in spheroid clusters of INS-1E cells with anti-SV2 antibody (left-hand panel). Spheroids of INS-1E cells infected with LacZ and SV2A, or SV2C encoding adenoviruses, were perifused with 2.5 mM glucose before stimulation with 15 mM glucose for 30 minutes. Values represent the mean±s.e. (n=3 per group) of one out of five independent experiments. Asterisks indicate a significant difference from levels in the LacZ control for corresponding time points at P<0.05.