Fig. 1. Characterization of cultured astroglia. (A) Confluent wild type (+/+) and AQP4-null (–/–) astroglial cell cultures showing similar morphology by phase-contrast microscopy (left panel) and similar GFAP expression (in red, middle panel). Right panel shows AQP4 immunofluorescence (green). Nuclei are counterstained blue using DAPI. (B) Proliferation of wild-type (AQP4^+/+) and AQP4-null (AQP4^–/–) astroglia. Cells were counted (eight wells per time point) on alternate days after plating 2.8x10⁴ astroglia/cm². (C) Percentage of astroglia adhering to poly-L-lysine at 4 hours after plating (ten wells per genotype). B and C show mean±s.e.m.; differences were not significant. Bar, 200 µm.