Fig. 1. TLS colocalized with RNA-containing particles in neuronal dendrites. (A) Mouse hippocampal neurons at culture day 21 (21 DIV) were labeled with SYTO14 (middle) and then immunostained with anti-TLS polyclonal antibody (left). Immunoreactive signals of endogenous TLS overlap with SYTO14-labeled RNA-containing particles (right). Scale bars, 10 µm in top panels, 5 µm in bottom panels. (B) TLS fused to GFP (FL) overlaps with RNA-containing particles stained with ethidium bromide (EtBr). Whereas GFP fused to an N-terminal fragment of TLS (N-TLS) is confined to the nucleus, that fused to the C-terminal fragment of TLS (C-TLS), which contains the RNA-binding domain, is observed in dendrites, where it is colocalized with RNA signals, even in the distal part of dendrites (over 80 µm from the soma). Arrowheads indicate representative TLS/RNA clusters. Bars, 2 µm in top three rows; 5 µm in bottom bottom. (C) Quantitative data obtained from B.