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Fig. 4. TLS associates with Nd1-L mRNA in mouse cortex polysome fraction. (A) Schematic representation of IP/RT-PCR. (B) Western blot to show specificity of anti-TLS monoclonal antibody used in this study. IgG: IP with IgG control; TLS: IP with anti-TLS monoclonal antibody; N: brain polysomal extract from TLS-null mouse cortex; P: brain polysomal extract from wild-type mouse cortex. (C) IP/RT-PCR to detect association of TLS with ß-actin mRNA (left) and Nd1-L mRNA (right). IgG: IP with IgG; anti-TLS: IP with anti-TLS antibody; no template: no template for PCR; cDNA: mouse cortex cDNA used as template. 2-log: 2-log DNA ladder; 100 bp: 100 bp DNA ladder.