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Fig. 1. Rqh1 is not required for bulk DNA replication. Wild-type (rqh1+) and rqh1{Delta} cells were arrested in G1 by nitrogen starvation at 25°C for 20 hours and released into nitrogen-rich medium to restart the cell cycle at the temperature of 25°C. Cells harvested at hourly intervals were processed for flow cytometry (A), and mitotic index was assessed by scoring bi-nucleate cells (n=200) (B). (C) Micrographs of DAPI-stained wild-type and rqh1{Delta} cells (lower panels), 9 hours after release from nitrogen starvation at 25°C. Bar, 10 µm.