Fig. 4. IFA analysis of NF-B localization in WT, IKK^–/–, IKKß^–/– and IKK^–/–ß^–/– MEFs infected with T. gondii for 24 hours. Double immunofluorescence labeling was performed with antibodies against p50 (A) and p65 (B), and anti-T. gondii SAG1. Translocation of p50 and p65 is observed in infected WT, IKK^–/– and IKKß^–/– cells but not in uninfected cells. A proportion of infected IKKß^–/– cells fails to show p65 translocation at a similar extent as WT and IKK^–/– cells (yellow arrow). Infected IKK^–/–ß^–/– cells do not display nuclear localization of p50 or p65. Bars, 20 µm.