Fig. 3. Dynamics of LEM2 during the cell cycle. (A) HeLa cells stably expressing hLEM2 were processed for indirect immunofluorescence using anti-phospho-histone-H3 antibody (pHistone3; red) and anti-V5 antibody (green). DNA was stained using Hoechst dye (blue). Representative images of defined cell-cycle stages are shown. (B) Cells at anaphase or different telophase stages, were stained for exogenous LEM2 with anti-V5 antibody (green) and for either LAP2, LBR or LAP2ß (red). DNA was stained with Hoechst (blue). Bars, 10 µm in (A) and 5 µm in (B).