Fig. 1. Phogrin-EGFP is effectively targeted to the insulin granules in ßTC3 cells. (A-C) Cells stably transfected with phogrin-EGFP were plated for 48 hours and then immunostained with an insulin antibody and an Alexa Fluor 546 secondary antibody. (D-F) Cells were co-transfected with phogrin-EGFP and proinsulin-ECFP (pseudocolored red). Measures were taken to minimize and correct for crossover fluorescence. Bar, 10 µm.