Fig. 5. Effects of cytochalasin D and jasplakinolide on granule mobility. (A-F) The effects of cytochalasin D (cyto D) and jasplakinolide (Jas) treatment on the actin network in ßTC3 cells. Cells were incubated with 10 µg/ml Cyto D or 1 µM Jas for 20 minutes at 37°C before being stained with Alexa Fluor 488-phalloidin. (G,H) The histogram of average velocity under basal condition (green), after Cyto D (G) or Jas (H) treatment (black), and after adding KCl (red). The inset bar graphs show the percentage of granules with speeds >0.4 µm/second, with the error bars representing s.e.m. *P<0.001, ^#P<0.01, ^&P<0.05 and ^P>0.1, as assessed by paired t-tests. Bar, 10 µm.