Fig. 6. Effect of nocodazole on granule mobility. (A-D) The effect of nocodazole (Noc) treatment on the microtubule network in ßTC3 cells. Cells were incubated with 10 mM Noc for 20 minutes at 37°C. They were then stained with -tubulin antibody and Alexa Fluor 488 secondary antibody. (E) The histograms of average velocity under basal condition (green), after Noc treatment (black), and after adding KCl (red). The inset bar graphs show the percentage of granules with speeds >0.4 µm/second, with the error bars representing s.e.m. ^&P<0.05 and ^P>0.1, as assessed by paired t-tests. Bar, 10 µm.