JCS -- Stanchi et al. 118 (24): 5899 Figure IG3

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Fig. 3. PINCH1fl/fl and PINCH1^–/– mouse embryonic fibroblasts (MEFs). (A) Western blot showing the absence of PINCH1 protein in the PINCH1^–/– MEFs and of the PINCH2 protein in both PINCH1fl/fl and PINCH1^–/– MEFs. To control the PINCH2 assay, a lysate from PINCH2^+/+ liver was also blotted. (B-E) Spreading of PINCH1fl/fl (B) and PINCH1^–/– (C) cells and of PINCH1^–/– cells transduced with EGFP-PINCH1 (D) and EGFP-PINCH2 (E), respectively. Cells were plated on tissue culture dishes for 16 hours in the presence of serum. Bar, 20 µm.