Fig. 5. Spreading of PINCH1fl/fl and PINCH1^–/– MEFs. The numbers in each frame indicate the time in minutes from the moment of plating. (A,B) Phase contrast images. PINCH1fl/fl (A) and PINCH1^–/– (B) MEFs were plated in DMEM on fibronectin-coated tissue culture dishes, incubated at 37°C and 5% CO₂, filmed at intervals with a CCD camera. Bar, 40 µm. (C,D) Immunofluorescence staining of PINCH1f/fl (C) and PINCH1^–/– cells (D). Cells were plated on fibronectin-coated glass, incubated for the time periods indicated and then fixed and stained. Bar, 20 µm.