Fig. 7. Acceleration of membrane fission by Ca²⁺ in inside-out excised membrane patches from INS-1 cells. Changes in fission pore conductance (G_p) were fit with single exponential decay functions. Bath application of a 100 nM free-Ca²⁺ buffer or 0.5 mM Ca²⁺ accelerated the kinetics of membrane fission. (A) Representative G_p traces with 0 mM Ca²⁺, 100 nM free-Ca²⁺ or 0.5 mM Ca²⁺ in the bath. (B) Fission pore time constants () were calculated by fitting the G_p traces with single exponential decay functions. Unlike what was seen with endocytotic vesicle size, the effect of Ca²⁺ on fission kinetics was not bimodal. *, P<0.05; **, P<0.01.