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Fig. 6. Role of MMP-3 in branching stimulated by senescent fibroblasts. (A) Morphology of representative collagen-embedded organoids after culture in the presence of conditioned medium from presenescent (Presen) mBF containing the indicated amounts of recombinant MMP-3. An organoid cultured with conditioned medium from senescent (Sen) (X-irradiated) mBF is shown for comparison. (B) Percentage of organoids that undergo primary (1' only) or higher level (secondary and tertiary; Side branching) branching in the presence of presenescent mBF-conditioned medium containing the indicated concentrations of recombinant MMP-3. The effect of conditioned medium from senescent (Sen) mBF is shown for comparison. (C) Proliferation of organoids, determined by DAPI fluorescence, in the presence of presenescent mBF-conditioned medium containing the indicated concentrations of recombinant MMP-3. The effect of conditioned medium from senescent (Sen) mBF is shown for comparison.