Fig. 5. TNFR2-mediated apoptosis is not dependent on endogenous production of TNF or other death-inducing ligands. PC60 R1R2 cells were treated with hTNF or its muteins as in Fig. 1 and the resulting amount of cell death determined. (A) Incubation in the absence or presence of 5 µg/ml hTNFR1 antagonizing monoclonal antibody htr-5. (B) Incubation in the absence or presence of 10 µg/ml cycloheximide (CHX).