Fig. 2. Subcellular distribution of HASPB-GFP fusion proteins as determined by confocal microscopy. Cells were grown on glass coverslips in the presence of 1 µg/ml doxicycline for 48 hours at 37°C. GFP-derived fluorescence was viewed with a Zeiss LSM 510 confocal microscope. (A) HASPB-N18-GFP; (B) HASPB-N18-GFP-palm; (C) HASPB-N18-GFP-myr/palm; (D) GFP.