Fig. 3. Quantitative analysis of cell surface localized HASPB-GFP fusion proteins using flow cytometry. (A) GFP-derived fluorescence (expression level). (B) APC-derived fluorescence (cell surface staining). Cells were incubated in the presence or absence of doxicycline as indicated followed by processing for FACS sorting. HASPB-N18-GFP, light and dark blue curves; HASPB-N18-GFP-palm, yellow and orange curves; HASPB-N18-GFP-myr/palm, light and dark green curves; GFP, light and dark red curves.