Fig. 7. (A) A genomic DNA segment that rescues the lsp1 phenotype. Boxes represent the open reading frames predicted by the exon-prediction software Green Genie. The sites of restriction enzyme digestion by which rescue was abolished are indicated on the top. The triangle shows the site of the plasmid insertion. The lsp1 phenotype was not rescued when the first or the last exon of the predicted gene was deleted (bottom). (B) Predicted primary structure of Lsp1. Phosphorylation sites are indicated. The sequences have been deposited in DDBJ/EMBL/GenBank with accession number AD194902.