Fig. 3. BFA blocks transit peptide cleavage. (A) Intracellular T. gondii expressing ACP-GFP were labeled with 35S amino acids for 30 minutes (pulse) then chased for 4 hours in medium containing the indicated concentrations (ng/ml) of BFA. GFP and Mic5 were immunoprecipitated, resolved by SDS-PAGE and detected by phosphorimaging. Transit peptide (t-GFP) and mature forms (m-GFP) of GFP as well as intermediate (i-mic5) and mature forms (m-mic5) of Mic5 are indicated. (B) Quantification of GFP (diamonds) and Mic5 (squares) processing calculated based on the maximum processing measured in the experiment shown in A.