Fig. 3. Mutation of the putative clathrin-binding box at amino acids 362-366 partially disrupts TOM1 association with clathrin. (A) Schematic diagram of TOM1 deletion constructs with or without mutation of ³⁶²Leu and ³⁶⁴Asp to Ala. (B) Mutation of ³⁶²LEDEF³⁶⁶ reduces the ability of TOM1 to pull down clathrin. Glutathione-sepharose-bound recombinant GST fusion proteins of the constructs depicted in A were incubated with A431 cytosol and analyzed as in Fig. 2B.