Fig. 6. Influence of PLC-1 mutants on adhesion to fibronectin. (A) Null cells were infected for 8 hours with wild-type vaccinia virus or virus containing point mutations Y771F, Y783F or Y1253F, or wild-type PLC-1, as described elsewhere (Sekiya et al., 2004). Null+ cells were used as control. Equal numbers of cells from each cell line were allowed to adhere on fibronectin-coated plates for 30 minutes at 37°C. , Null cells with control virus; , Null cells with wild-type PLC-1 virus; , Null cells with Y771F virus; , Null cells with Y783F virus; , Null cells with Y1253F virus. (B) Null cells or Null cells expressing either wild-type PLC-1 or a PLC-1 mutant that contains loss of function mutations in the N- and C-terminal SH2 domains [N+C SH2^- (Ji et al., 1999)] were allowed to adhere to fibronectin-coated plates for 30 minutes at 37°C. , Null cells; , Null+ cells expressing wild-type PLC-1; , Null+ cells expressing N+C SH2^- PLC-1.