Fig. 7. JAM-A expression causes morphological changes in directionally migrating cells and in cell groups. In (A), confluent cell monolayers were scratch-wounded and incubated for an additional 24 hours. In (B), cells were seeded at high density and incubated for 2 hours to allow the formation of small groups of contacting cells. Finally, cells were stained with crystal violet (Phase contrast), FITC-phalloidin (F-actin), and anti-vinculin mAb hVIN-1 (Vinculin).