Fig. 11. Nesprin-2 epitopes are abundant in skeletal muscle. N3 and CH3 epitopes were localised to the sarcomere in a striated pattern and both colocalized in a punctate manner with titin T12 at the Z-line (A-D). N3 was also distributed around the nucleus (inset in C) and was present at the A/I junction where it colocalized with titin-A/I (E-G). The localization pattern for CH3 was essentially the same as that observed for N3 but this epitope was particularly abundant over the A/I junction where it colocalized with titin A/I (H). Nesprin-2 antibodies did not colocalize with myomesin at the M-line (N3 shown, I-K) confirming the Z-line/A/I junction distribution. The N2 antibody recognised the NE and nucleus, and was localized in a punctate pattern throughout the sarcomere in a pattern consistent with N3 and CH3, only weaker (arrow in L). Bar, 5 µm.