Fig. 10. siRNA-mediated depletion of migfilin and its effect on ß-catenin organization. Human HT-1080 cells that were transfected with the migfilin siRNA (lane 2) or the control small RNA (lane 1) were analyzed by western blotting with monoclonal anti-migfilin antibody (clone 43) (A), monoclonal anti-actin antibody (B) or a polyclonal anti-ß-catenin antibody (C). (D-K) HT-1080 cells transfected with the control RNA (D, F, H and J) or the migfilin siRNA (E, G, I and K) were double stained with mouse monoclonal anti-migfilin antibody (clone 43) (H-K) and rabbit-polyclonal anti-ß-catenin antibody (F and G). The differential interference contrast (DIC) (D and E) and immunofluorescent (F-K) images were recorded using a Leica DM R fluorescence microscope equipped with a Hamamatsu ORCA-ER digital camera. Bar, 8 µm.