Fig. 6. Expression and schematic representation of the wild-type and mutant forms of migfilin and their localization to cell-cell and cell-ECM adhesions. (A) Expression of GFP-tagged wild-type and mutant forms of migfilin. HyCat cells were transfected with expression vectors encoding the wild-type and mutant forms of migfilin as indicated in the figure. The cell lysates (15 µg/lane) were analyzed by western blotting with an anti-GFP antibody. N-ter, residues 1-85; PR-LIM1-3, residues 85-373; LIM1-3, residues 176-373; LIM1N, residues 205-373; C243, C291 and C306, migfilin bearing C243G, C291G or C306G point mutation. C306(s), migfilin(s) bearing the C306G point mutation. (B) Summary of the localization of the wild-type and mutant forms of migfilin to cell-cell and cell-ECM adhesions, based on the results presented in this paper.