Fig. 8. LIM2 is essential for migfilin localization to adherens junctions. HaCat cells were transfected with the expression vectors encoding GFP-LIM1N (A), GFP-C243 (C), GFP-C291 (E) and GFP-C306(s) (G). The cells were stained with the anti-ß-catenin antibody as indicated (B, D, F and H). Notice that a small amount of GFP-tagged C306(s) mutant was detected in b-catenin-rich adherens junctions (G and H, arrows). Bar, 8 µm.