Fig. 3. Binding of peptide-specific antibody K7 to the wild type and mutant N-cadherin-Fc fusion protein. (a) The mutations W2G and D134A in N-cadherin Fc, dimerised via Fc, were tested singly or in combination and compared with results from the wild-type (Wt) molecule. Calcium was present in the assay at 1.25 mM. (b) N-cadherin-Fc mutant W2G was pre-equilibrated with varying levels of Ca²⁺, which were maintained throughout the assay. (c) Monomeric N-cadherin-Fc was tested in the presence of 1.25 mM Ca²⁺.