Fig. 2. Metaphase delay during mitosis 16 in pim mutant embryos. Time-lapse in vivo imaging was used for the analysis of progression through the sixteenth round of mitosis during Drosophila embryogenesis in pim1 mutant (pim1) and pim+ (pim+) sibling embryos expressing red fluorescent histone H2AvD-mRFP and a green fluorescent microtubule-binding protein. Selected frames of merged images are shown. Numbers above and below the frames indicate the time in minutes relative to the last metaphase frame which was set to zero. Bar in upper right frame, 5 µm.