Fig. 6. Ang-2-dependent PMA-mediated destabilization of co-culture spheroids. Co-culture spheroids of siRNA-treated or untreated HUVECs with HUASMCs were incubated in spinner culture with the indicated combination of reagents for 4 hours and endothelial-cell detachment was quantified by automated image analysis of CD31 whole-mount-stained spheroids. PMA treatment leads to massive denudation of the surface monolayer of the untreated endothelial cells within 4 hours (A,C) but not of the monolayer of Ang-2-siRNA-silenced endothelial cells (A,E). ^**, P<0.001 compared with control.