Fig. 5. Immunofluorescence of BSF line Bp2T7/ARL1 grown in the absence (-Tet) and presence (+Tet) of tetracycline for 24 and 36 hours. All cells are co-stained with DAPI (shown in blue). (A) ER-localized BiP staining (green). (B) Lysosomal marker, p67 (red). (C) Heavy chain of T. brucei clathrin (green). Bar, 10 µm.