Fig. 5. Centrosomal assembly of BBS6 is not dependent on polymerized microtubules or the dynein-dynactin molecular motor. (A) IMCD3 cells were either mock-treated (no drug) or treated with nocodazole for 1 hour and allowed to recover for 0 or 10 minutes. Cells were then co-stained with antibodies to BBS6 or -tubulin (green) and -tubulin (red). (B) IMCD3 cells were transiently transfected with myc-tagged p50-dynamitin, which inhibits dynein-dynactin motor function. Cells were then stained with an anti-myc antibody (red) and costained with antibodies to BBS6, PCM1 or -tubulin (green). Arrows denote centrosomal BBS6 and -tubulin signals from p50-dynamitin-transfected cells, and arrowheads denote centrosomal BBS6, PCM1 and -tubulin in untransfected cells. Note the dispersed PCM1 signal in transfected cells.