Fig. 2. FISH analysis of metaphase chromosomes. Metaphase spreads were hybridized with probes detecting the genomic fragments (green) and the vector sequences (red). (A) Loci encoding HMW-glutenin subunits in wild-type wheat (arrowheads). (B) Transgene loci (orange, hybridizing with both genomic and vector sequences) and gene loci (green, arrowheads) of loci encoding HMW-glutenin subunits in line E. Bar, 10 µm.