Fig. 7. Disappearance of cytoplasmic HMW-glutenin-encoding RNA at the onset of silencing. Sections from wax-embedded endosperm of line E were hybridized with an antisense probe for the coding region of 1Ax1 and detected with Cy3 (red). The nuclei were counterstained with DAPI. The images comprise only the nucleus and the cytoplasm immediately around it. (A) 10 dpa. HMW-glutenin-encoding RNA can be seen inside the nucleus and in the cytoplasm around it. (B) 14 dpa. RNA is still visible inside the nucleus, whereas it has disappeared from the cytoplasm. Bar, 10 µm.