Fig. 8. Detection of siRNAs corresponding to the 1Ax1 coding region. Total seed RNA of the wild-type (WT) and line E extracted before (8 dpa) and after (14 dpa) the onset of silencing was hybridized with an antisense probe for the coding region of 1Ax1. Two RNA bands of
22 bp and 25 bp (which are indicative of PTGS) are visible in line E at 14 dpa (lane 4).