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Fig. 4. Effect of nak1/orb3-167 on the microtubule cytoskeleton. Exponentially growing wild-type (WT) and temperature-sensitive nak1/orb3-167 cells were transferred from 25°C to 35°C. Samples were taken before and 30 minutes and 60 minutes after a shift from 25°C to 35°C, fixed and stained with an antibody against tubulin. Bars, 10 µm.