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Fig. 6. Localization of Nak1/Orb3. (A) Localization of Nak1/Orb3-GFP in vivo. Living cells expressing Nak1/Orb3 tagged with GFP under the endogenous promoter. Arrows point at dots at the nuclei that might correspond to the spindle pole body. (B) Colocalization of Nak1/Orb3-GFP and Sad1-DsRed in live cells. (C) Colocalization of Nak1/Orb3-GFP and actin. Cells expressing GFP-tagged Nak1/Orb3 were fixed and double stained with antibodies against actin (red) and GFP (green). Overlay of both channels is shown in yellow. Bars, 10 µm.