Fig. 6. Loss of the 6ß4 integrin does not affect stratification and differentiation of keratinocytes. (A-F) Cryosections of skin from two-day-old ß4^flox/flox; K14-Cre mice processed for indirect immunofluorescence and visualized by confocal microscopy. Primary antibodies are against proteins indicated in the left lower corner of each image, and colors are coded according to FITC or Texas Red secondary antibodies. In all panels, the epidermis is at the top and the dermis at the bottom. (G) Semi-quantitative RT-PCR showing that there were no significant changes in the level of transcripts for a range of basal (6 integrin, Dsg-2 and -3 and the 2 chain of Ln-5) and suprabasal (Dsg-1 and envoplakin) cell markers in NMK-1(+) and (–) cells.