Fig. 5. Proteasome inhibition increases the phosphorylation of MAP kinase p42/p44. HUVECs and ECV-304 cells were serum-starved overnight and treated with MG-262 (600 nM). Phosphorylation of MAP kinase p42/p44 was analyzed by immunoblot after 0, 90 and 180 minutes of treatment (upper panels). Corresponding amounts of total p42 are shown in lower panels.