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Fig. 4. In vivo association of trichoplein with K8/18. (A) Detection of trichoplein in HeLa, T24 and DJM-1 cells. Lysates from HeLa, T24 and DJM-1 cells were stained with CBB, detected with trichoplein antibody by immunoblotting ({alpha}-trichoplein), or immunostained with trichoplein antibody preabsorbed by GST-trichoplein protein (absorbed). Trichoplein antibody specifically recognized bands of about 61 kDa in samples. Molecular size markers are shown on the left. (B) Interaction of trichoplein with K8/18 in vivo. Immunoprecipitates were prepared from lysates of HeLa, T24 and DJM-1 cells using control rabbit IgG and trichoplein antibody. Each precipitate was subjected to immunoblot analysis with trichoplein, K8 or K18 antibody.