Fig. 2. Immunocytochemistry of infected erythrocytes. Membranes were immunolabeled with quantum dots for anti-band 3 mAb (red) and parasite nuclei were labeled with Hoechst 33258 (blue). These images are the result of 3D reconstruction and deconvolution. (A) Parasitized AA erythrocytes surrounded by uninfected erythrocytes. (B) Parasitized CC erythrocytes surrounded by uninfected erythrocytes. Note the abnormal membrane appearance of uninfected CC erythrocytes. Comparisons of erythrocyte shapes at early, mid, and late stages of parasitized AA (C-E) and CC erythrocytes (F-H). Bars, 5 µm (A,B); bars, 1 µm (C-H).