Fig. 10. CD98 association with integrin ß1 induces focal adhesion formation. IMCD populations expressing the different constructs indicated were plated onto collagen I-coated coverslips for 1 hour and cells were subsequently stained with Alexa 594-conjugated phalloidin to visualize the cytoskeleton (upper panel) and anti-phosphopaxillin antibody to identify activated focal adhesions (middle panel). The lower panels show merged images. Bar, 50 µm.