Fig. 3. The transmembrane domain of CD98 is required to enhance cell adhesion and migration. (A) The IMCD cell populations transfected with the constructs indicated were plated onto 96-well plates coated with collagen I at the concentrations indicated for 1 hour in serum-free medium. Adherent cells were then stained with crystal violet, lysed and the OD measured. Data represent the mean±s.d. of quadruplicate samples/cell population. (B) IMCD cell populations were plated in transwell dishes coated with 1 µg/ml collagen I and migration was evaluated 4 hours after plating. The values indicate the mean±s.d. of three independent experiments. Differences in cell adhesion and cell migration between CD98 or CD98 chimeras and vector control cells (*), or between C69T98E98 and CD98 (#), or CD98 and C98T98E98 (+) were significant with P<0.05.