Fig. 8. CD98 association with integrin ß1 induces FAK and AKT phosphorylation. The different cell populations transfected with the CD98 constructs indicated were serum starved for 12 hours, trypsinized and left in suspension or replated on 10 µg/ml collagen I for 10 or 30 minutes. Equal amounts of cell lysate were separated by 10% SDS-PAGE and transferred to nitrocellulose. The membranes were immunoblotted with antibodies to phospho-FAK (p-FAK), total FAK, phospho-AKT (p-AKT), total AKT, phospho-ERK (p-ERK) and ERK.