Fig. 9. CD98-induced cell adhesion and migration is mediated by PI 3-kinase. The cell populations transfected with the CD98 constructs indicated were serum starved for 12 hours and then kept untreated or treated with Wortmannin (100 nM) or LY294002 (5 µM) for 4 hours. The cells were trypsinized and their adhesion (A) and migration (B) analyzed as described in Fig. 3. To verify that Wortmannin and LY294002 inhibited PI3-K activity, cells cultured with or without the inhibitors were lysed 10 minutes after plating on collagen I and immunoblotted for phospho-AKT and total AKT (40 µg lane). Differences in adhesion and migration between untreated and Wortmannin- or LY294002-treated cells (*) were significant with P<0.05.