Fig. 1. Expression and subcellular localization of TRPV channels. Rat TRPV1 (A) and murine TRPV2 to TRPV6 (B-F) were C-terminally fused to YFP, and plasmids were transiently transfected in HEK293 cells. Cells were imaged by confocal laser-scanning microscopy 1 day after transfection. The pinholes were adjusted to obtain optical sections with a thickness of 0.6 µm. Typical expression patterns of the different TRPV channels from three to five independent transfections are shown. Bar, 10 µm.