Fig. 2. Subcellular localization of coexpressed TRPV channel subunits. (A-I) Different combinations of TRPV channels tagged with either CFP or YFP were coexpressed in HEK293 cells and sequentially imaged by confocal laser-scanning microscopy in the same cell. For each coexpression experiment, the correlation coefficient between CFP and YFP fluorescence intensities (r²) was estimated by pixel-based image analysis. Shown are representative data of three independent transfections. Bars, 10 µm.